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Detection of anti-Leishmania infantum antibodies by immunofluorescence

Presentation Advantages

Method: Immunofluorescence on a slide

Analysis: Detection of anti-Leishmania infantum antibodies using a fluorescein-labelled antiglobulin (Antigen of Leishmania infantum zymodeme Mon 1)

Sample: plasma, serum

Preparation: 2 hours

Reading: 20 minutes

Storage: 18 months at 2°C – 8°C

Presentation: 20 tests

Reliability: Immunofluorescence is the reference technique for the assay of anti-leishmania antibodies. This assay is standardized using reference sera.

FluoleishTM uses the reference method (IF) as recommended by the OIE (World Organisation for Animal Health) to determine the canine leishmania status of an animal.

Thanks to a quantitative assay, FluoleishTM can be used to determine and monitor the animal's leishmania immune status.

FluoleishTM uses the most commonly encountered strain in the Mediterranean basin; numerous European Laboratories appreciate it for its readability.

Why use Fluoleish TM ?

                          Faced with a poorly specific clinical presentation and a long asymptomatic phase, immunofluorescence is the most sensitive diagnostic technique, which is recommended by the World Organisation for Animal Health(1) and is considered as being the reference method.

Antibodies can be detected from the first few weeks of infestation and persist for a long time. During the clinical progression of the disease, the immunofluorescence reaction varies in intensity, making it possible to assess the efficacy or inefficacy of treatment (2).

When use Fluoleish TM ?

In the event of indicative clinical signs, FluoleishTM can be used to pinpoint the diagnosis of clinical leishmaniasis.

In endemic areas, FluoleishTM can be used for the early detection of asymptomatic dogs. (1)
On return from endemic areas, FluoleishTM can be used to detect a potential seroconversion, with 2 tests performed 3 months apart.

FluoleishTM is a quantitative method and can therefore be used to monitor antibody levels to check the efficacy of treatment or assess the need for a new treatment.

Protocole d'utilisation de Fluoleish TM:


Références bibliographiques:

(2) BOURDOISEAU G. Parasitologie clinique du chien. 2000, Ed. NEVA, 325-36  
(1) OIE, Manual of standards for diagnostic tests and vaccines, 2000, X.1, 803-812