Speed Duo FeLV/FIV

Rapid combined detection of FeLV antigens and anti-FIV antibodies

Presentation

Advantages

Method: Membrane Immunochromatography

Analysis: Combined detection of P27 FeLV capsid antigen and antibodies directed against the GP40, an envelope glycoprotein of FIV

Sample: Whole blood with anticoagulant, plasma or serum

Preparation: 2 minutes

Reading: 15 minutes

Storage: 24 months at room temperature (+2°C to +30°C)

Presentations: 6 tests, 20 tests, 50 tests

Reliability: Compared with the reference methods:

Viral isolation (FeLV)	Western Blot (FIV)
Sens. 94,7%	Sens. 96,3%
Spec. 99,2%	Spec. 98,9%

The technology of the Speed Duo FeLV/FIV, based on the detection of soluble viral antigens for FeLV and on GP40 antibodies for FIV, makes it highly sensitive.

Quick and easy, Speed Duo FeLV/FIV can be performed in-practice, in one step and in only 15 minutes.

SpeedDuo FeLV/FIV can be stored for 24 months at room temperature, between 2°C and 30°C.

Why to use Speed Duo FeLV/FIV?
Infection with the feline leukemia virus (FeLV) causes variable and poorly specific symptoms, which make laboratory testing essential for the diagnosis. The differential diagnosis includes other viral immunosuppressive diseases, as FIV and feline coronavirus. Moreover, the clinical phase is preceded by an asymptomatic phase. Early detection and correct differential diagnosis may be critical for the course of the disease. Regular screening of cats infected with FeLV is considered as the best means of fighting against the virus ⁽²⁾. Depending on the regions affected, studies show variable prevalences from 1% to 20%. The detection of soluble viral antigens makes it possible to determine an animal's status even when there is a very low viral load in the blood specimen (early infection, transient viraemia, sequestrated infection). The mean seroprevalance of FIV ranges from 4% to 66% and is around 40% in certain populations of at risk cats (stray cats, unneutered/unsprayed cats...). Detection of specific antibodies directed against FIV makes it possible to determine the precise immune status of the cat.

Why to use Speed Duo FeLV/FIV?

Infection with the feline leukemia virus (FeLV) causes variable and poorly specific symptoms, which make laboratory testing essential for the diagnosis. The differential diagnosis includes other viral immunosuppressive diseases, as FIV and feline coronavirus.

Moreover, the clinical phase is preceded by an asymptomatic phase. Early detection and correct differential diagnosis may be critical for the course of the disease.

Regular screening of cats infected with FeLV is considered as the best means of fighting against the virus ⁽²⁾. Depending on the regions affected, studies show variable prevalences from 1% to 20%.

The detection of soluble viral antigens makes it possible to determine an animal's status even when there is a very low viral load in the blood specimen (early infection, transient viraemia, sequestrated infection).

The mean seroprevalance of FIV ranges from 4% to 66% and is around 40% in certain populations of at risk cats (stray cats, unneutered/unsprayed cats...). Detection of specific antibodies directed against FIV makes it possible to determine the precise immune status of the cat.

When to use Speed Duo FeLV/FIV?
Speed Duo FeLV/FIV is well adapted to the regular monitoring of the FeLV and the FIV status. It can be proposed at the annual visit, before surgery and more commonly following suspicion of exposure to the virus. In multi-cat environments, the determination of the status of new arrivals can be used to prevent non-infected cats from being exposed to the virus. An animal's status for FeLV can be tested 28 days after potential exposure to the virus (interval between infection and viraemia). An animal's status for FIV can be tested 60 days after potential exposure to the virus (interval between infection and presence of detectable antibodies). Speed Duo FeLV/FIV is recommended prior to vaccination against FeLV, to prevent vaccination failure ⁽²⁾.

When to use Speed Duo FeLV/FIV?

Speed Duo FeLV/FIV is well adapted to the regular monitoring of the FeLV and the FIV status. It can be proposed at the annual visit, before surgery and more commonly following suspicion of exposure to the virus.

In multi-cat environments, the determination of the status of new arrivals can be used to prevent non-infected cats from being exposed to the virus.

An animal's status for FeLV can be tested 28 days after potential exposure to the virus (interval between infection and viraemia).

An animal's status for FIV can be tested 60 days after potential exposure to the virus (interval between infection and presence of detectable antibodies).

Speed Duo FeLV/FIV is recommended prior to vaccination against FeLV, to prevent vaccination failure ⁽²⁾.

Instruction for use

Bibliography:
(1) HARTMANN K. et coll. Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection. Journal of Feline Medicine and Surgery, 2007, 9, 439-445
(2) LEVY J. et coll. Report of the American Association of Feline Practitioners, 2005
(3) CACHON T., CHUZEL T. Epidémiologie, pathogénie et symptômes de la PIF. Point Vétérinaire, 2005, 254, 18-21

Websites
ABCD-VETS : European Advisory Board for Cat Diseases

The European Advisory Board on Cat Diseases (ABCD) aims to issue guidelines on the prevention and management of feline infectious disease in Europe.

Recommendations of ABCD Vet for FeLV
Recommendations of ABCD Vet for FIV

AAFP : American association of Feline practitionners

The American Association of Feline Practitioners improves the health and well-being of cats by supporting high standards of practice, continuing education.

Recommendations of AAFP for FeLV and FIV