Method: Membrane Immunochromatography

Analysis: Detection of anti Ehrlichia canis antibodies and of circulating antibodies (lgG) directed against Leishmania infantum

Sample: Whole blood with anticoagulant, serum or plasma

Preparation: 2 minutes

Reading: 15 minutes

Storage: 24 months at room temperature (2°C to 30°C)

Presentations: 6 tests, 20 tests

Reliability:

Canine Leishmaniosis is due to the proliferation in the macrophages of Leishmania infantum. The clinical phase of the disease precents an asymptomatique stage that lasts from a few months to years ^{(1 )}. The disease is transmitted by sand flies: the phlebotomes.

Canine Ehrlichiosis is due to the infection of the animal's monocytes by a bacteria: Ehrlichia canis. After an acute stage, most often unnoticed, illness evolves towards a long subclinical stage of several months. The vector of Ehrlichia is a tick: Rhipicephalus sanguineus.

Depending on the region, seroprevalence in dogs varies from 0% to 60%. In endemic areas, as the Mediterranean basin, 25 to 30% of carriers are asymptomatic⁽⁴⁾; ehrlichiosis is also highly prevalent ⁽⁴⁾. Coinfections are common and differential diagnosis is difficult.

For either of the 2 diseases, the detection of circulating antibodies by immunochromatographie is more sensitive than the direct highlighting of the parasite.

In the presence of suggestive symptoms, Speed Duo Leish/Ehrli can be used to clarify the etiological diagnosis and to help the practitioner with the differential diagnosis.

In endemic areas, using Speed Duo Leish/Ehrli regularly is strongly recommended to enable early detection of the diseases in asymptomatic dogs ⁽³⁾.

Returning from an endemic area, Speed Duo Leish/Ehrli can be used to screen for a potential seroconversion